Hplc column selection guide pdf

Hplc column selection guide pdf
This selection guide is primarily focused on Agilent’s small molecule HPLC columns; please see publication 5990-9384EN for information about our family of biocolumns or 5990-7994EN and 5990-7995EN for more detailed information about GPC/SEC columns.
4 I. INTRODUCTION LOCATING AND CORRECTING THE PROBLEM A systematic approach to identifying the problem is the best approach to trou-bleshooting your HPLC system.
To find the columns that are most similar to the one selected above, sort the column database below by F s so that the smallest F s values are listed first. To find the columns that are most different from the one selected above, sort the column database below by F …

HPLC method development and validation play important role in the discovery, development and manufacture of agro chemicals , pharmaceutical products.
Specialty HPLC and UPLC Columns Performance Benefits and Bonding Performance Standards Application Standards BioSuite DEAE, SP, and CM (HPLC) Protein-Pak
Agilent Maintenance Guides On this page you will find manuals for the extensive Agilent Instrument Ranges of HPLC, GC, MS and Spectroscopy products. As well as routine maintenance procedures, you will also find exploded instrument diagrams and quick lists to ensure you choose the correct parts for your repair and maintenance operations.
Protein Analysis Shodex HPLC Column Selection Guide Shodex CAPTURE THE ESSENCE ® For application data and product information visit: www.shodex.de
HPLC Column Selection Guide We prepared useful table that you can select suitable TCI HPLC column from analyte and separation mode. Please click the button below to check it.
Capcell Pak columns provide reversed-phase, normal-phase and HILLC (Reverse of reversed-phase) modes ion-exchange separation modes. The following guide will help chromatographers to choose a
Bio Columns Selection Guide LC Columns and Accessories for Biomolecules >> View product information and application notes. Thermo Scientific Chromatography Columns and Consumables 2016-2017 4-004 LC Columns for Biomolecules HPLC Phases for Biomolecules Silica-based Size Exclusion Chromatography Phases Silica-based Reversed Phase and Ion Exchange Phases Silica-based …
Description. Agilent PLRP-S is a rigid macroporous styrene/divinylbenzene (PS/DVB) HPLC phase with outstanding chemical and physical stability. PLRP-S HPLC media are inherently hydrophobic and reproducibile and do not require a bonded alkyl chain such as C8 or C18 to confer hydrophobicity.
GC Column Selection Guide Step 2 – Column I.D. The current range of commercially available capillary column internal diameters enables the balancing of two factors: efficiency (number of theoretical plates) and sample capacity (amount of any one sample component that can be applied to the column without causing the desired sharp peak to overload). Optimizing one of these factors …
Column and Cartridge Selection Guide BIO RAD . PROTEIN PURIFICATION RESIN AND COLUMNS Bio-Rad has a comprehensive chromatography column and cartridge portfolio for high-quality protein purification. Columns and cartridges are available for preparative affinity, ion exchange, size exclusion, and mixed-mode chromatography for the most common and important lab-scale techniques. In addition, HPLC



Gas Chromatography Liner Selection Guide

To choose the right column KNAUER developed a Column Selection Guide with focus on reversed phase columns. This guide will lead you and help you to find a column that fits best for your application. This guide will lead you and help you to find a column that fits best for your application.
Agilent offers the widest range of fast HPLC/UHPLC wide-pore columns. So you have the fl exibility to create methods with So you have the fl exibility to create methods with maximum resolution, whether you have a 400, 600, or 1200 bar instrument.
HPLC COLUMN PRODUCT GUIDE 2018-2019 • Widest Range of RPC Selectivities at High Efficiency • Excellent General Purpose Phases • Specific Columns for Acids and Bases • Superior Columns for LC/MS • Ideal Columns for Diastereomers/Geometric Isomers • Specialized Column for Highly Aqueous Mobile Phase Reversed-Phase Chromatography (RPC) Reversed-Phase Chromatography …
Tony Edge (Thermo Scientific) shares some great general hints and tips for HPLC column selection. Read the literature It is very rare that a separation has never been performed before and so checking the literature, refereed journals, trade magazines and even dare I say it the manufacturer’s webs sites is an excellent use of resources
The Essential CHROMacademy Guide Mobile Phase Optimization Strategies for Reversed Phase HPLC . Webcast Notes •Type your questions in the “Submit Question” box, located below the slide window •You can enlarge the slide window at any time by clicking on the “Enlarge Slides” button, located below the presentation window –the slides will advance automatically throughout the event

ChiraDex Chiral Description ChiraDex Chiral columns are cartridge columns that use a novel manufacturing process to bond b-cyclodextrin to spherical 5 µm silica by
Aminex HPLC columns, which are packed with a polymer-based matrix, are the research and industry standard for the analysis of carbohydrates, alcohols,
In general, HPLC system is consisted of pump, injector, separation column, detector, and data processor(CDS). And it is need to configure the suitable system for columns.
USLC™ Column Selection & Mobile Phase Adjustment Guide RESTEK USLC™ Ultra Selective Liquid Chromatography™ Innovative Chromatography Products www.restek.com. Ultra Selective Liquid Chromatography™ (USLC™) technology is the directed application of selectivity—the most influential factor affecting peak separation, or resolution—to provide the practicing chromatographer with the
The scope of this guide has been extended where necessary to include LC/MS column and instrument troubleshooting. The focus of the bulk of the causes of problems and their corrective
To order the Application CD. The CHIRAL TECHNOLOGIES Applications Guide CD contains over 1,100 examples of chiral separations by liquid chromatography and includes a copy of the CHIRAL TECHNOLOGIES Products and Services Catalog.
In “LC word”, the former means that using the same column with different conditions (mobile phase/ temperature etc…) and the latter case means to change the LC column. There are different LC column packing materials (base material) available. Moreover using different modifications (addition of chemical compounds on the surface of the gel), a wide variety of LC column can be prepared.

The largest portfolio of Fast LC columns, and a broad family of phases across all particle sizes for exceptional flexibility and scalability Whether you are performing conventional or ultra-fast chromatography, separating biomolecules,
HPLC Analysis of Biomolecules Technical Guide chromatography Part of Thermo Fisher Scientific. HPLC Analysis of Biomolecules 2 Resources for Chromatographers Available now, the Thermo Scientific Chromatography Columns and Consumables 2010/2011 catalog. Beyond this catalog, our Chromatography team shares its extensive expertise through our web-based Chromatography …
onto the column. Detailed in this selection guide are the main types of liners with a description of their relative merits, as well as the process of selecting the correct liner for a particular application. Key Words Liner, focus Abstract The liner serves an important function in allowing a sample which is injected in the liquid phase to pass into the gaseous phase and onto the GC column
This GC column selection guide discusses the basics of separation and teaches you how to choose the right GC column! Resolution is the goal of every chromatographer, but how much resolution is enough? Practically speaking, we need enough reten- tion to get sharp symmetrical peaks that are baseline resolved from each other, but not too much retention, where retention times are too long and
LC Column Selection LC Columns and Accessories >> HPLC Stationary Phase Column Selection Before beginning a new analysis, consider the physical and chemical properties of the analyte(s), the mode of analysis and how the analyte(s) will interact with the surface of the chromatographic phase . To aid column selection, the following guide may be useful . Non-polar. LC Columns and Accessories
LC Columns and Sample Prep Navigator Simplify and speed selection. Easily pinpoint the right LC columns and sample preparation options for achieving the greatest accuracy in your chromatographic analysis using multiple search methods.
Columns HPLC Columns Column Selection Guide Silica Gel Type Reversed Phase High-Speed Analysis Conventional Analysis With Certificate of Compliance for Validation Wider pH Range Stronger Retentivity Wider pH Range Stronger Retentivity Higher Cost-Performance Strong Retentivity of Basic Compounds With Certificate of Compliance for Validation Rapid Separation and Wider pH Range …

HPLC Column Selection Guide Qmx

LC Columns and Consumables Catalog Access the latest version of our Chromatography Columns and Consumables Catalog to ensure that you’re optimizing your applications! Our enhanced online version features a robust applications search tool, access to method development guides, application notebooks, and much more.
COLUMN SUGGESTED CONDITIONS Need improved selectivity for organic anions including nucleic acids. ZirChrom®-SAX Use at neutral pH with phosphate and NaCl.
HPLC Column Selection Guide. Carbon Load Bonding Type — — OH — X —O — — Bonded Phase Bonded Phase Bonded Phase Bonded Phase Bonded Phase Bonded Phase
UHPLC+ Solutions Ultrahigh Performance LC (UHPLC) enables LC separations on columns with sub-3 um particles and therefore features shorter LC analysis times and higher resolution compared to conventional HPLC.
HPLC column selection guide is presented below. This selection is, neither in terms of manufacturers nor in terms of their products, a complete list, and the accuracy of the data is not


Column length is typically predicated by the resolution required from a “traditional” HPLC system and in this sense we mean systems which have not been designed or adapted to minimize extra column volume or cannot achieve system back pressures above 400 bar.
The applications guide highlights some common, new and novel performance demonstrations of SGE’s GC column technologies. Use them to understand your next analysis or to evaluate your next purchase! Use them to understand your next analysis or to evaluate your next purchase!
Organic Chemistry I – Lab. High Performance Liquid Chromatography HPLC is characterized by the use of high pressure to push a mobile phase solution through a column of stationary phase allowing separation of complex mixtures with high resolution.
HPLC Column Selection by Ph.Eur. Listing The European Pharmacopoeia (Ph. Eur.), of the Council of Europe is a pharmacopoeia, listing a wide range of active substances and excipients used to prepare pharmaceutical products in Europe. It includes general and specific monographs that give quality standards for all the main medicines used in Europe. All medicines sold in the 38 Member States of
HPLC Column Selection Guide Column Parameters Internal Diameter An important parameter of a HPLC column is the internal diameter (ID) as this directly influences the
The Supelco HPLC Column Selection Guide is designed to assist you in choosing the right column for your application based on the features, benefi ts and specifi cations of some of our most popular phases. This guide provides one of the most diverse sets of HPLC phases available from any company. We not only emphasize our newest family of columns, Ascentis™, but also cover our 25-year
4 Classification by Support Material 1Classification of Shimadzu HPLC Columns The solid supports of packing materials for HPLC are classified into the following two groups with the following features.

Column Selection for HPLC Method Development LCGC



For more than 40 years, liquid chromatography columns from KNAUER have been well-known for their high quality and performance. In the field of analytical HPLC columns we can offer a solution for nearly any analytical separation task.
LC COLUMN SELECTION Characterisation of C18 Phases. hydrophobicity The strength of hydrophobic interaction can be measured by the retention of neutral (non-polar) molecules. The k values (retention factors) for a neutral species, for a given C18 phase, will give an indication of the surface area and surface coverage (ligand density) of the silica. The percentage of carbon in the material is a
The Supelco HPLC Column Selection Guide is designed to assist you in choosing the right column for your application based on the features, benefits and specifications of some of our most popular phases. This guide provides one of the most diverse sets of HPLC phases available from any company. We not only emphasize our newest family of columns, Ascentis™, but also cover our 25-year history


Dionex Product Selection Guide Dionex – PDF Catalogs

ProteCol™ HPLC Columns turn imagination into results Imagine being able to successfully separate chelating compounds with sensitivity and speed
The variety and number of bioanalytical HPLC applications continue to grow rapidly. Agilent Technologies’ goal is to offer products that can be used to
Obelisc® LC Columns with Liquid Separation Cell Technology 1.01MB PDF (6 pages) Newcrom HPLC columns. New generation of HPLC column format 0.9 Mb PDF (4 pages)
Using Accucore HPLC columns excellent separations can be achieved in shorter times The examples on this page show how by increasing flow rates while maintaining efficiency, and …
The key to good column selection is knowing the dominant characteristics required from the chosen column set which drive the separation of your analyte types, or covering the widest selectivity range possible if screening unknown or unfamiliar analytes.
approach to HPLC column selection based upon complementary decisions regarding bonded-phase and particle physical characteristics. First, the authors used knowledge of the sample components, particularly molecular structure information, as a guide in choosing bonded-phase characteristics. Second, they used an analysis of the separation goals in choosing column and particle physical
Waters UPLC, UHPLC, and HPLC Column Selection and Mobile-Phase Guide [ www.waters.com ] High retentivity for basic compounds Excellent peak shape at elevated pH Good universal column choice for a wide variety of compounds Stable across a wide pH range For separations at high temperatures (80 °C) High retentivity for polar organic compounds and metabolites Balanced retention …

HPLC Column Product guide zirchrom.com

26 analytical hplc hplc columns introduction Directions for Using the Column Chart The chart lists the columns in descending order of hydrophobic capacity (H) .
Column Selection Guide 13 Column selection guide (Biochromatography) For separatiion or molecular weight determination of sugars For separation of polar compounds
4 www.ace-hplc.com Practical Implications The characteristics of the column and of samples in general, lead to a recommendation to start method development with a mobile phase in the pH 2-3
Ordering Info HPLC/UHPLC Column Phase Selection Chart Specifically designed for successful and reproducible method development and transfer Use for highest performance compared to …
1 inTroDuCTion Every Phenomenex HPLC column is a precision product which, though delicate, will provide excellent performance, reproducibility and column lifetime if cared for properly.


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